Tag

Part:BBa_K3946001

Designed by: Fiona Bunn   Group: iGEM21_Edinburgh   (2021-10-19)


Car9 silica tag (C)

A short silica-binding tag to add to the C-terminal of a protein using JUMP assembly, including a short glycine-rich linker (GSGGGS). The tag facilitates immobilisation to silica surfaces, enabling enzyme immobilisation or purification using silica-based spin columns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None